NCERT : BIOLOGY : BIO TECHNOLOGY 2

QUESTIONS : 45

TIME : 25 MIN

1 / 45

1) Which of the following is not a feature of the plasmids?

A) Circular structure

B) Single-stranded

C) Independent replication

D) Transferable

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2) Use of bioresources by multinational companies and organisations without authorisation from the concerned country and its people is called ?

A) bioexploitation.

B) biodegradation

C) biopiracy

D) bio-infringement

3 / 45

3) Restriction endonucleases are

A) present in mammalian cells for degradation of DNA.

B) synthesized by bacteria

C) used in genetic engineering

D) used for in vitro DNA synthesis

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4) The two polypeptides of human insulin are linked together by (NCERT XII:211)

A) hydrogen bonds

B) phosphodiester bond.

C) covalent bond

D) disulphide bridges

5 / 45

5) In India, the organisation responsible for assessing the safety of introducing genetically modified organisms for public use is (NCERT XII : 213)

A) Indian Council of Medical Research (ICMR)

B) Council for Scientific and Industrial Research (CSIR)

C) Genetic Engineering Appraisal Committee (GEAC).

D) Research Committee on Genetic Manipulation (RCGM)

6 / 45

6) Two bacteria found to be very useful in genetic engineering experiments are

A) Rhizobium and Diplococcus

B) Escherichia and Agrobacterium.

C) Nitrobacter and Azotobacter

D) Nitrosomonas and Klebsiella

7 / 45

7) Genetic engineering is possible, because (NCERT XII:194)

A) we can cut DNA at specific sites by endonucleases like DNase I

B) the phenomenon of transduction in bacteria is well understood

C) we can see DNA by electron microscope

D) restriction endonucleases purified from bacteria can be used in vitro

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8) Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes? (NCERT XII:200)

A) pBR322

B) Retrovirus

C) λ phage

D) Ti plasmid

9 / 45

9) Which one of the following bacteria has found extensive use in genetic engineering work in plants?

A) Xanthomonas citri

B) Agrobacterium tumefaciens

C) Bacillus coagulens

D) Clostridium septicum

10 / 45

10) In gel electrophoresis, separated DNA fragments can be visualized with the help of ?

A) ethidium bromide in UV radiation

B) ethidium bromide in infrared radiation.

C) acetocarmine in bright blue light

D) acetocarmine in UV radiation

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11) The linking of antibiotic resistance gene with the plasmid vector became possible with __?

A) endonucleases

B) DNA ligase

C) DNA polymerase

D) exonucleases

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12) The process of RNA interference (RNAi) has been used in the development of plants resistant to __?

A) viruses

B) nematodes

C) fungi

D) insects.

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13) The process of separation and purification of expressed protein before marketing is called (NCERT XII:204)

A) postproduction processing

B) downstream processing

C) bioprocessing

D) upstream processing

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14) The first clinical gene therapy was given for treating (NCERT XII:211)

A) diabetes mellitus

B) rheumatoid arthritis

C) adenosine deaminase deficiency.

D) chicken pox

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15) Plasmid has been used as vector because __?

A) it is circular DNA which have capacity to join to eukaryotic DNA

B) it can move between prokaryotic and eukaryotic cells

C) both ends show replication

D) it has antibiotic resistance gene.

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16) Human insulin is being commercially produced from a transgenic species of __?

A) Saccharomyces

B) Escherichia

C) Mycobacterium

D) Rhizobium

17 / 45

17) Which of the following is not a component of downstream processing?

A) Purification

B) Separation

C) Preservation

D) Expression

18 / 45

18) A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using ?

A) Taq polymerase

B) ligase

C) polymerase III

D) EcoRI

19 / 45

19) The Taq polymerase enzyme is obtained from ?

A) Bacillus subtilis

B) Thermus aquaticus

C) Pseudomonas putida

D) Thiobacillus ferroxidans.

20 / 45

20) The linking of antibiotic resistance gene with the plasmid vector became possible with (NCERT XII:197)

A) endonucleases

B) DNA polymerase

C) DNA ligase

D) exonucleases

21 / 45

21) A ‘new’ variety of rice was patented by a foreign company, though such varieties have been present in India for a long time. This is related to ____?

A) Basmati

B) Co-667

C) Sharbati Sonora

D) Lerma Rojo

22 / 45

22) Which one of the following techniques made it possible to genetically engineer living organisms?

A) Hybridization

B) Heavier isotope labelling

C) X-ray diffraction

D) Recombinant DNA techniques

23 / 45

23) Golden rice is a transgenic crop of the future with the following improved trait?

A) high lysine (essential amino acid) content

B) insect resistance

C) high protein content

D) high vitamin-A content.

24 / 45

24) Which one of the following techniques made it possible to genetically engineer living organisms? (NCERT XII :194)

A) Heavier isotope labelling

B) Hybridization

C) Recombinant DNA techniques

D) X-ray diffraction

25 / 45

25) Following statements describe the characteristics of the enzyme restriction endonuclease. Identify the incorrect statement.(NCERT XII:196)

A) The enzyme binds DNA at specific sites and cuts only one of the two strands.

B) The enzyme cuts DNA molecule at identified position within the DNA.

C) The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.

D) The enzyme recognises a specific palindromic nucleotide sequence in the DNA.

26 / 45

26) Which one of the following is a case of wrong matching ?

A) Micropropagation – In vitro production of plants in large numbers

B) Vector DNA – Site for tRNA synthesis

C) Callus – Unorganized mass of cells produced in tissue culture

D) Somatic – Fusion of two hybridization diverse cells

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27)

A) D

B) B

C) A

D) C

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28) Which body of the Government of India regulates GM research and safety of introducing GM organisms for public services?

A) Genetic Engineering Approval Committee

B) Research Committee on Genetic Manipulation

C) Bio-safety committee

D) Indian Council of Agricultural Research

29 / 45

29) Genetic engineering is possible, because ___?

A) restriction endonucleases purified from bacteria can be used in vitro

B) we can see DNA by electron microscope

C) the phenomenon of transduction in bacteria is well understood

D) we can cut DNA at specific sites by endonucleases like DNase I

30 / 45

30) Choose the correct pair from the following?

A) Polymerases - Break the DNA into fragments

B) Ligases - Join the two DNA molecules

C) Nucleases - Separate the two strands of DNA

D) Exonucleases - Make cuts at specific positions within DNA

31 / 45

31) Restriction endonucleases are

A) present in mammalian cells for degradation of DNA.

B) used for in vitro DNA synthesis

C) synthesized by bacteria

D) used in genetic engineering

32 / 45

32) A gene whose expression helps to identify transformed cell is known as (NCERT XII:199)

A) plasmid

B) vector

C) structural gene

D) selectable marker.

33 / 45

33) Stirred-tank bioreactors have been designed for (NCERT XII:204)

A) ensuring anaerobic conditions in the culture vessel

B) availability of oxygen throughout the process.

C) purification of the product

D) addition of preservatives to the product

34 / 45

34) Which one is a true statement regarding DNA polymerase used in PCR? (NCERT XII:203)

A) It remains active at high temperature.

B) It is used to ligate introduced DNA in recipient cells.

C) It serves as a selectable marker.

D) It is isolated from a virus.

35 / 45

35) What is antisense technology? (NCERT XII:209)

A) RNA polymerase producing DNA

B) When a piece of RNA that is complementary in sequence is used to stop expression of a specific gene

C) Production of somaclonal variants in tissue cultures

D) A cell displaying a foreign antigen used for synthesis of antigens

36 / 45

36) Maximum number of existing transgenic animals is of (NCERT XII:212)

A) mice

B) pig.

C) cow

D) fish

37 / 45

37) Which one of the following is now being commercially produced by biotechnological procedures ___?

A) Nicotine

B) Morphine

C) Quinine

D) Insulin

38 / 45

38)

A) A

B) D

C) B

D) C

39 / 45

39) Gel electrophoresis is used for (NCERT XII:198)

A) separation of DNA fragments according to their size.

B) construction of recombinant DNA by joining with cloning vectors

C) isolation of DNA molecules

D) cutting of DNA into fragments

40 / 45

40) Biolistics (gene-gun) is suitable for ___?

A) constructing recombinant DNA by joining with vectors

B) disarming pathogen vectors

C) transformation of plant cells

D) DNA fingerprinting.

41 / 45

41) What is antisense technology ___?

A) Production of somaclonal variants in tissue cultures

B) RNA polymerase producing DNA

C) A cell displaying a foreign antigen used for synthesis of antigens

D) When a piece of RNA that is complementary in sequence is used to stop expression of a specific gene

42 / 45

42) Which one of the following is now being commercially produced by biotechnological procedures? (NCERT XII:211)

A) Morphine

B) Quinine

C) Insulin

D) Nicotine

43 / 45

43) Bacillus thuringiensis forms protein crystals which contain insecticidal protein. This protein___?

A) binds with epithelial cells of midgut of the insect pest ultimately killing it

B) is activated by acid pH of the foregut of the insect pest

C) does not kill the carrier bacterium which is itself resistant to this toxin.

D) is coded by several genes including the gene cry

44 / 45

44) The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of (NCERT XII:200)

A) insertional inactivation of alpha galactosidase in non-recombinant bacteria.

B) insertional inactivation of alpha galactosidase in recombinant bacteria

C) inactivation of glycosidase enzyme in recombinant bacteria

D) non-recombinant bacteria containing beta galactosidase

45 / 45

45) Which vector can clone only a small fragment of DNA?

A) Yeast artificial chromosome

B) Bacterial artificial chromosome

C) Plasmid

D) Cosmid

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NCERT : BIOLOGY : BIO TECHNOLOGY 2

TIME : 25 MIN

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