NCERT : BIOLOGY : BIO TECHNOLOGY 2

QUESTIONS : 45

TIME : 25 MIN

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1) Which one of the following is a case of wrong matching ?

A) Micropropagation – In vitro production of plants in large numbers

B) Vector DNA – Site for tRNA synthesis

C) Somatic – Fusion of two hybridization diverse cells

D) Callus – Unorganized mass of cells produced in tissue culture

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2) Bacillus thuringiensis forms protein crystals which contain insecticidal protein. This protein___?

A) is coded by several genes including the gene cry

B) binds with epithelial cells of midgut of the insect pest ultimately killing it

C) is activated by acid pH of the foregut of the insect pest

D) does not kill the carrier bacterium which is itself resistant to this toxin.

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3) Use of bioresources by multinational companies and organisations without authorisation from the concerned country and its people is called ?

A) bio-infringement

B) bioexploitation.

C) biodegradation

D) biopiracy

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4) Which one is a true statement regarding DNA polymerase used in PCR? (NCERT XII:203)

A) It serves as a selectable marker.

B) It remains active at high temperature.

C) It is used to ligate introduced DNA in recipient cells.

D) It is isolated from a virus.

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5) Genetic engineering is possible, because (NCERT XII:194)

A) we can cut DNA at specific sites by endonucleases like DNase I

B) restriction endonucleases purified from bacteria can be used in vitro

C) the phenomenon of transduction in bacteria is well understood

D) we can see DNA by electron microscope

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6) The first clinical gene therapy was given for treating (NCERT XII:211)

A) rheumatoid arthritis

B) adenosine deaminase deficiency.

C) chicken pox

D) diabetes mellitus

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7) What is antisense technology ___?

A) When a piece of RNA that is complementary in sequence is used to stop expression of a specific gene

B) Production of somaclonal variants in tissue cultures

C) A cell displaying a foreign antigen used for synthesis of antigens

D) RNA polymerase producing DNA

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8) In gel electrophoresis, separated DNA fragments can be visualized with the help of ?

A) ethidium bromide in UV radiation

B) acetocarmine in bright blue light

C) ethidium bromide in infrared radiation.

D) acetocarmine in UV radiation

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9) Which of the following is not a feature of the plasmids?

A) Independent replication

B) Circular structure

C) Single-stranded

D) Transferable

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10) Stirred-tank bioreactors have been designed for (NCERT XII:204)

A) ensuring anaerobic conditions in the culture vessel

B) availability of oxygen throughout the process.

C) purification of the product

D) addition of preservatives to the product

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11) Following statements describe the characteristics of the enzyme restriction endonuclease. Identify the incorrect statement.(NCERT XII:196)

A) The enzyme recognises a specific palindromic nucleotide sequence in the DNA.

B) The enzyme binds DNA at specific sites and cuts only one of the two strands.

C) The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.

D) The enzyme cuts DNA molecule at identified position within the DNA.

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12) The two polypeptides of human insulin are linked together by (NCERT XII:211)

A) covalent bond

B) hydrogen bonds

C) phosphodiester bond.

D) disulphide bridges

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13) Which of the following is not a component of downstream processing?

A) Separation

B) Purification

C) Preservation

D) Expression

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14) Which one of the following is now being commercially produced by biotechnological procedures ___?

A) Morphine

B) Insulin

C) Quinine

D) Nicotine

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15) The process of separation and purification of expressed protein before marketing is called (NCERT XII:204)

A) bioprocessing

B) downstream processing

C) postproduction processing

D) upstream processing

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16) Which one of the following bacteria has found extensive use in genetic engineering work in plants?

A) Agrobacterium tumefaciens

B) Xanthomonas citri

C) Bacillus coagulens

D) Clostridium septicum

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17) Maximum number of existing transgenic animals is of (NCERT XII:212)

A) pig.

B) mice

C) fish

D) cow

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18) Which one of the following techniques made it possible to genetically engineer living organisms?

A) Hybridization

B) Recombinant DNA techniques

C) X-ray diffraction

D) Heavier isotope labelling

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19)

A) D

B) A

C) B

D) C

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20) Which one of the following is now being commercially produced by biotechnological procedures? (NCERT XII:211)

A) Quinine

B) Insulin

C) Nicotine

D) Morphine

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21) The process of RNA interference (RNAi) has been used in the development of plants resistant to __?

A) viruses

B) fungi

C) insects.

D) nematodes

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22) Which one of the following techniques made it possible to genetically engineer living organisms? (NCERT XII :194)

A) Hybridization

B) Recombinant DNA techniques

C) X-ray diffraction

D) Heavier isotope labelling

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23) What is antisense technology? (NCERT XII:209)

A) A cell displaying a foreign antigen used for synthesis of antigens

B) RNA polymerase producing DNA

C) Production of somaclonal variants in tissue cultures

D) When a piece of RNA that is complementary in sequence is used to stop expression of a specific gene

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24) Gel electrophoresis is used for (NCERT XII:198)

A) isolation of DNA molecules

B) separation of DNA fragments according to their size.

C) construction of recombinant DNA by joining with cloning vectors

D) cutting of DNA into fragments

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25) Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes? (NCERT XII:200)

A) pBR322

B) Ti plasmid

C) Retrovirus

D) λ phage

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26) In India, the organisation responsible for assessing the safety of introducing genetically modified organisms for public use is (NCERT XII : 213)

A) Research Committee on Genetic Manipulation (RCGM)

B) Indian Council of Medical Research (ICMR)

C) Council for Scientific and Industrial Research (CSIR)

D) Genetic Engineering Appraisal Committee (GEAC).

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27) A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using ?

A) Taq polymerase

B) ligase

C) polymerase III

D) EcoRI

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28) Two bacteria found to be very useful in genetic engineering experiments are

A) Nitrosomonas and Klebsiella

B) Rhizobium and Diplococcus

C) Nitrobacter and Azotobacter

D) Escherichia and Agrobacterium.

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29) The linking of antibiotic resistance gene with the plasmid vector became possible with (NCERT XII:197)

A) endonucleases

B) exonucleases

C) DNA polymerase

D) DNA ligase

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30) Genetic engineering is possible, because ___?

A) we can cut DNA at specific sites by endonucleases like DNase I

B) we can see DNA by electron microscope

C) the phenomenon of transduction in bacteria is well understood

D) restriction endonucleases purified from bacteria can be used in vitro

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31) Golden rice is a transgenic crop of the future with the following improved trait?

A) insect resistance

B) high protein content

C) high lysine (essential amino acid) content

D) high vitamin-A content.

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32) Restriction endonucleases are

A) used in genetic engineering

B) synthesized by bacteria

C) present in mammalian cells for degradation of DNA.

D) used for in vitro DNA synthesis

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33) Biolistics (gene-gun) is suitable for ___?

A) DNA fingerprinting.

B) constructing recombinant DNA by joining with vectors

C) disarming pathogen vectors

D) transformation of plant cells

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34)

A) A

B) B

C) C

D) D

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35) Plasmid has been used as vector because __?

A) it can move between prokaryotic and eukaryotic cells

B) both ends show replication

C) it has antibiotic resistance gene.

D) it is circular DNA which have capacity to join to eukaryotic DNA

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36) The Taq polymerase enzyme is obtained from ?

A) Thiobacillus ferroxidans.

B) Thermus aquaticus

C) Bacillus subtilis

D) Pseudomonas putida

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37) A gene whose expression helps to identify transformed cell is known as (NCERT XII:199)

A) plasmid

B) structural gene

C) selectable marker.

D) vector

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38) The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of (NCERT XII:200)

A) insertional inactivation of alpha galactosidase in recombinant bacteria

B) insertional inactivation of alpha galactosidase in non-recombinant bacteria.

C) non-recombinant bacteria containing beta galactosidase

D) inactivation of glycosidase enzyme in recombinant bacteria

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39) A ‘new’ variety of rice was patented by a foreign company, though such varieties have been present in India for a long time. This is related to ____?

A) Co-667

B) Lerma Rojo

C) Sharbati Sonora

D) Basmati

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40) The linking of antibiotic resistance gene with the plasmid vector became possible with __?

A) DNA ligase

B) exonucleases

C) endonucleases

D) DNA polymerase

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41) Human insulin is being commercially produced from a transgenic species of __?

A) Saccharomyces

B) Rhizobium

C) Escherichia

D) Mycobacterium

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42) Which vector can clone only a small fragment of DNA?

A) Bacterial artificial chromosome

B) Cosmid

C) Yeast artificial chromosome

D) Plasmid

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43) Restriction endonucleases are

A) synthesized by bacteria

B) present in mammalian cells for degradation of DNA.

C) used in genetic engineering

D) used for in vitro DNA synthesis

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44) Choose the correct pair from the following?

A) Polymerases - Break the DNA into fragments

B) Exonucleases - Make cuts at specific positions within DNA

C) Ligases - Join the two DNA molecules

D) Nucleases - Separate the two strands of DNA

45 / 45

45) Which body of the Government of India regulates GM research and safety of introducing GM organisms for public services?

A) Research Committee on Genetic Manipulation

B) Indian Council of Agricultural Research

C) Genetic Engineering Approval Committee

D) Bio-safety committee

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NCERT : BIOLOGY : BIO TECHNOLOGY 2

TIME : 25 MIN

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