NCERT : BIOLOGY : BIO TECHNOLOGY 2

QUESTIONS : 45

TIME : 25 MIN

1 / 45

1) Which of the following is not a feature of the plasmids?

A) Circular structure

B) Independent replication

C) Single-stranded

D) Transferable

2 / 45

2) The two polypeptides of human insulin are linked together by (NCERT XII:211)

A) disulphide bridges

B) hydrogen bonds

C) phosphodiester bond.

D) covalent bond

3 / 45

3) The first clinical gene therapy was given for treating (NCERT XII:211)

A) rheumatoid arthritis

B) chicken pox

C) adenosine deaminase deficiency.

D) diabetes mellitus

4 / 45

4) The process of RNA interference (RNAi) has been used in the development of plants resistant to __?

A) insects.

B) nematodes

C) viruses

D) fungi

5 / 45

5) Restriction endonucleases are

A) synthesized by bacteria

B) used for in vitro DNA synthesis

C) present in mammalian cells for degradation of DNA.

D) used in genetic engineering

6 / 45

6) Following statements describe the characteristics of the enzyme restriction endonuclease. Identify the incorrect statement.(NCERT XII:196)

A) The enzyme cuts DNA molecule at identified position within the DNA.

B) The enzyme binds DNA at specific sites and cuts only one of the two strands.

C) The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.

D) The enzyme recognises a specific palindromic nucleotide sequence in the DNA.

7 / 45

7) In India, the organisation responsible for assessing the safety of introducing genetically modified organisms for public use is (NCERT XII : 213)

A) Research Committee on Genetic Manipulation (RCGM)

B) Council for Scientific and Industrial Research (CSIR)

C) Indian Council of Medical Research (ICMR)

D) Genetic Engineering Appraisal Committee (GEAC).

8 / 45

8) The Taq polymerase enzyme is obtained from ?

A) Thiobacillus ferroxidans.

B) Pseudomonas putida

C) Bacillus subtilis

D) Thermus aquaticus

9 / 45

9) A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using ?

A) ligase

B) polymerase III

C) EcoRI

D) Taq polymerase

10 / 45

10) Which body of the Government of India regulates GM research and safety of introducing GM organisms for public services?

A) Indian Council of Agricultural Research

B) Bio-safety committee

C) Genetic Engineering Approval Committee

D) Research Committee on Genetic Manipulation

11 / 45

11) Which one of the following is now being commercially produced by biotechnological procedures ___?

A) Nicotine

B) Morphine

C) Insulin

D) Quinine

12 / 45

12) Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes? (NCERT XII:200)

A) Retrovirus

B) Ti plasmid

C) λ phage

D) pBR322

13 / 45

13) Which one of the following bacteria has found extensive use in genetic engineering work in plants?

A) Bacillus coagulens

B) Agrobacterium tumefaciens

C) Xanthomonas citri

D) Clostridium septicum

14 / 45

14) What is antisense technology? (NCERT XII:209)

A) RNA polymerase producing DNA

B) When a piece of RNA that is complementary in sequence is used to stop expression of a specific gene

C) A cell displaying a foreign antigen used for synthesis of antigens

D) Production of somaclonal variants in tissue cultures

15 / 45

15) Which vector can clone only a small fragment of DNA?

A) Plasmid

B) Cosmid

C) Bacterial artificial chromosome

D) Yeast artificial chromosome

16 / 45

16) Biolistics (gene-gun) is suitable for ___?

A) disarming pathogen vectors

B) DNA fingerprinting.

C) constructing recombinant DNA by joining with vectors

D) transformation of plant cells

17 / 45

17) Two bacteria found to be very useful in genetic engineering experiments are

A) Escherichia and Agrobacterium.

B) Rhizobium and Diplococcus

C) Nitrosomonas and Klebsiella

D) Nitrobacter and Azotobacter

18 / 45

18) Maximum number of existing transgenic animals is of (NCERT XII:212)

A) pig.

B) cow

C) mice

D) fish

19 / 45

19) Choose the correct pair from the following?

A) Ligases - Join the two DNA molecules

B) Exonucleases - Make cuts at specific positions within DNA

C) Nucleases - Separate the two strands of DNA

D) Polymerases - Break the DNA into fragments

20 / 45

20)

A) C

B) A

C) B

D) D

21 / 45

21) Stirred-tank bioreactors have been designed for (NCERT XII:204)

A) availability of oxygen throughout the process.

B) addition of preservatives to the product

C) purification of the product

D) ensuring anaerobic conditions in the culture vessel

22 / 45

22) The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of (NCERT XII:200)

A) non-recombinant bacteria containing beta galactosidase

B) insertional inactivation of alpha galactosidase in non-recombinant bacteria.

C) insertional inactivation of alpha galactosidase in recombinant bacteria

D) inactivation of glycosidase enzyme in recombinant bacteria

23 / 45

23) Gel electrophoresis is used for (NCERT XII:198)

A) isolation of DNA molecules

B) cutting of DNA into fragments

C) separation of DNA fragments according to their size.

D) construction of recombinant DNA by joining with cloning vectors

24 / 45

24) Use of bioresources by multinational companies and organisations without authorisation from the concerned country and its people is called ?

A) biopiracy

B) biodegradation

C) bio-infringement

D) bioexploitation.

25 / 45

25) A gene whose expression helps to identify transformed cell is known as (NCERT XII:199)

A) vector

B) structural gene

C) selectable marker.

D) plasmid

26 / 45

26)

A) C

B) A

C) D

D) B

27 / 45

27) Plasmid has been used as vector because __?

A) it can move between prokaryotic and eukaryotic cells

B) it is circular DNA which have capacity to join to eukaryotic DNA

C) it has antibiotic resistance gene.

D) both ends show replication

28 / 45

28) Which one of the following is a case of wrong matching ?

A) Somatic – Fusion of two hybridization diverse cells

B) Micropropagation – In vitro production of plants in large numbers

C) Vector DNA – Site for tRNA synthesis

D) Callus – Unorganized mass of cells produced in tissue culture

29 / 45

29) Which one of the following techniques made it possible to genetically engineer living organisms? (NCERT XII :194)

A) Recombinant DNA techniques

B) Hybridization

C) Heavier isotope labelling

D) X-ray diffraction

30 / 45

30) In gel electrophoresis, separated DNA fragments can be visualized with the help of ?

A) acetocarmine in bright blue light

B) acetocarmine in UV radiation

C) ethidium bromide in UV radiation

D) ethidium bromide in infrared radiation.

31 / 45

31) Golden rice is a transgenic crop of the future with the following improved trait?

A) high protein content

B) high lysine (essential amino acid) content

C) insect resistance

D) high vitamin-A content.

32 / 45

32) Restriction endonucleases are

A) present in mammalian cells for degradation of DNA.

B) synthesized by bacteria

C) used for in vitro DNA synthesis

D) used in genetic engineering

33 / 45

33) Bacillus thuringiensis forms protein crystals which contain insecticidal protein. This protein___?

A) binds with epithelial cells of midgut of the insect pest ultimately killing it

B) is coded by several genes including the gene cry

C) does not kill the carrier bacterium which is itself resistant to this toxin.

D) is activated by acid pH of the foregut of the insect pest

34 / 45

34) Which one is a true statement regarding DNA polymerase used in PCR? (NCERT XII:203)

A) It serves as a selectable marker.

B) It remains active at high temperature.

C) It is isolated from a virus.

D) It is used to ligate introduced DNA in recipient cells.

35 / 45

35) The linking of antibiotic resistance gene with the plasmid vector became possible with (NCERT XII:197)

A) DNA polymerase

B) DNA ligase

C) endonucleases

D) exonucleases

36 / 45

36) Which of the following is not a component of downstream processing?

A) Expression

B) Separation

C) Preservation

D) Purification

37 / 45

37) A ‘new’ variety of rice was patented by a foreign company, though such varieties have been present in India for a long time. This is related to ____?

A) Basmati

B) Co-667

C) Lerma Rojo

D) Sharbati Sonora

38 / 45

38) The process of separation and purification of expressed protein before marketing is called (NCERT XII:204)

A) upstream processing

B) bioprocessing

C) downstream processing

D) postproduction processing

39 / 45

39) Genetic engineering is possible, because ___?

A) restriction endonucleases purified from bacteria can be used in vitro

B) we can cut DNA at specific sites by endonucleases like DNase I

C) the phenomenon of transduction in bacteria is well understood

D) we can see DNA by electron microscope

40 / 45

40) The linking of antibiotic resistance gene with the plasmid vector became possible with __?

A) exonucleases

B) DNA ligase

C) endonucleases

D) DNA polymerase

41 / 45

41) Which one of the following is now being commercially produced by biotechnological procedures? (NCERT XII:211)

A) Nicotine

B) Morphine

C) Quinine

D) Insulin

42 / 45

42) Genetic engineering is possible, because (NCERT XII:194)

A) we can cut DNA at specific sites by endonucleases like DNase I

B) we can see DNA by electron microscope

C) the phenomenon of transduction in bacteria is well understood

D) restriction endonucleases purified from bacteria can be used in vitro

43 / 45

43) Human insulin is being commercially produced from a transgenic species of __?

A) Mycobacterium

B) Saccharomyces

C) Escherichia

D) Rhizobium

44 / 45

44) Which one of the following techniques made it possible to genetically engineer living organisms?

A) X-ray diffraction

B) Heavier isotope labelling

C) Hybridization

D) Recombinant DNA techniques

45 / 45

45) What is antisense technology ___?

A) RNA polymerase producing DNA

B) When a piece of RNA that is complementary in sequence is used to stop expression of a specific gene

C) A cell displaying a foreign antigen used for synthesis of antigens

D) Production of somaclonal variants in tissue cultures

Your score is

The average score is 58%

0%

Exit

NCERT : BIOLOGY : BIO TECHNOLOGY 2

TIME : 25 MIN

RECENT POSTS